The attack took place in the Colle Oppio park, close to the Colosseum, after the woman had been at a bar near the central Termini train station.
The woman is reportedly unable to remember the rape, but recalls being punched in the face and having her mobile phone and purse stolen. A passer-by came to her aid and took the Australian to the San Giovanni hospital, where she is recovering from concussion and injuries to her face.
She was in the Italian capital for a study trip and had been due to leave for Paris on Tuesday, Italian media reported however she is expected to stay in the hospital for up to a month.
According to Il Corriere, police are looking for a 30-year-old Romanian man who was seen leaving the bar together with the victim in CCTV footage, however they suspect a second man may have been involved in the rape.
On Monday, Rome police arrested three people for the rape and beating of another woman, a 48-year-old Brazilian.
The attack took place on September 25th near the Tuscolana train station, when the woman was reportedly kidnapped by two men and a woman, who beat her, tied her up and raped her.
She passed out from the violence but was able to escape while her attackers were sleeping, and went to hospital where she reported the attack. Two men, aged 27 and 31, and a woman were arrested on Monday evening in connection with the rape, Rai News reported.